Reversible dissociation of fatty acid synthetase from lactating rat mammary gland
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چکیده
منابع مشابه
Fatty Acid Synthetase from Lactating Rat Mammary Gland
Fatty acid synthetase has been purified from lactating rat mammary gland and some of its physical and kinetic properties studied. This synthetase is an asymmetrical multienzyme complex of molecular weight 478,000 which readily dissociates into approximately half-molecular weight subunits. The sedimentation and diffusion coefficients of the native enzyme are 12.9 set X lo-l3 and 2.56 cm2 se@ X l...
متن کاملFatty acid synthase from lactating rat mammary gland.
Homogeneous preparations of fatty acid synthetase from mammary glands of lactating rats were used to prepare an antiserum and a y-globulin fraction was isolated from the antiserum. The antiserum and the y-globulin fraction were shown to be specific against the fatty acid synthetase multienzyme complex. Addition of the y-globulin to homogenate fractions of lactating rat mammary glsnds, resulted ...
متن کاملThe absolute rate of fatty acid synthesis by mammary gland slices from lactating rats.
Mammary gland utilizes a combination of substrates in vivo. To determine if and how the substrates presented to the gland alter milk fat synthesis, slices of mammary gland of lactating rats were incubated with combinations of glucose, L-lactate, and pyruvate. Uptakes and conversions of uniformly and specifically labeled substrates to CO2 and fatty acid were measured. The absolute rate of fatty ...
متن کاملRetinol esterification by mammary gland microsomes from the lactating rat.
Because vitamin A in milk is largely present as esterified retinol while blood plasma predominantly contains unesterified retinol, experiments were conducted to determine whether membranes from the lactating mammary gland are able to synthesize retinyl esters in vitro. When microsomes from rats lactating for 7 to 14 days were incubated with [(3)H]retinol dispersed in dimethylsulfoxide, some [(3...
متن کاملMolecular weight and subunit size of fatty acid synthetase from rabbit mammary gland.
Fatty acid synthetase purified from the mammary gland of the rabbit has a mol. wt. of 968000 as determined by gel filtration. The enzyme gave one band, corresponding to a mol.wt. of approx. 35000, on polyacrylamide-gel electrophoresis in the presence of sodium dodecyl sulphate and phenylmethanesulphonyl fluoride.
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1970
ISSN: 0306-3283
DOI: 10.1042/bj1200031p